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John Arne Nesheim

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Please contact John Arne Nesheim for more information

Ploidy Work Station (PWS)

Localizes, segments, measures and classifies cell nuclei in monolayers

PWS automates the handling and analysis of nucleic images for DNA ploidy. The software package localizes the nuclei, focuses the camera and segments the nuclei, separating it from the surrounding image. Each cropped, focused nucleic image is then categorized according to cell type and DNA content based on pre-defined criteria. PWS Classifier displays the nuclei galleries to the analyst, who can manually go through and inspect individual nuclei, if necessary. A histogram showing the optical density of all nuclei in the tumor sample is automatically created and classified as diploid, tetraploid or aneuploid.

PWS supports scanning of several sections simultaneously, and stores the images in TIF and .nuc format. PWS is designed for a ­Windows platform.

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Chief Editor: Prof. Håvard E. Danielsen
Copyright Oslo University Hospital. Visiting address: The Norwegian Radium Hospital, Ullernchausséen 64, Oslo. Tel: 22 78 23 20